Martinsburg is a town located in Audrain County, in the state of Missouri (MO), United States. Nestled in the West North Central division of the Midwest region, it covers an area of 0.3 square miles (1.0 km²) and sits at an elevation of 807 ft. With a population of approximately 326 residents, Martinsburg offers a peaceful, rural atmosphere.
The town's population is composed of about 51.84% males and 48.16% females, and it has a population density of 253.85 people per square mile (484.11/km²). Martinsburg lies at a latitude of 39.1 and a longitude of -91.65, operating in the UTC-6.
Locals reflect a blend of American ancestry, with roots primarily in United States (12%), followed by English (5.8%), German (41.7%), and Scotch-Irish (0%) heritage. The town is identified by the ZIP code 65264.